Effect of hydrogen on expression of nuclear factor E2-related factor 2 during endotoxin-induced oxi-dative injury to macrophages
10.3760∕cma.j.issn.0254-1416.2016.09.028
- VernacularTitle:氢对内毒素致巨噬细胞氧化损伤时Nrf2表达的影响
- Author:
Gang LIU
;
Hongguang CHEN
;
Keliang XIE
;
Yonghao YU
- Keywords:
Hydrogen;
Endotoxins;
Macrophages;
Oxidative stress
- From:
Chinese Journal of Anesthesiology
2016;36(9):1150-1152
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effect of hydrogen on the expression of nuclear factor E2?related factor 2 ( Nrf2 ) during endotoxin?induced oxidative injury to macrophages. Methods Normally cultured Raw264.7 cells were divided into 4 groups ( n=36 each) using a random number table: control group (group C), hydrogen?rich saline group ( group H), endotoxin group ( group E) and endotoxin plus hydrogen?rich saline group (group EH). In E and EH groups, endotoxin was added with the final concentration of 1 μg∕ml. Hydrogen?rich saline ( hydrogen concentration 0. 06 mmol∕L) was added in H and EH groups. All the cells were incubated for 48 h. At 6, 24 and 48 h of incubation, cells were collect?ed to detect the activity of reactive oxygen species ( ROS) , and cells were collected and proteins extracted for determination of superoxide dismutase ( SOD) and catalase ( CAT) activities and Nrf2 expression by Western blot. Results Compared with group C, the ROS activity was significantly increased, the levels of SOD and CAT were significantly decreased, and the expression of Nrf2 was significantly up?regulated in E and EH groups (P<0.05), and no significant change was found in the parameters mentioned above in group H (P>0.05). Compared with group E, the ROS activity was significantly decreased, the levels of SOD and CAT were significantly increased, and the expression of Nrf2 was significantly up?regulated in group EH (P<0.05). Conclusion Hydrogen can attenuate endotoxin?induced oxidative injury to macro?phages, and the mechanism may be related to up?regulated expression of Nrf2.
