The relationship between expression of endogenous cystathionine beta-synthase and hydrogen sulfide in rectal mucosa tissue and the activeness of ulcerative colitis
10.3760/cma.j.jssn.1673-4904.2016.12.003
- VernacularTitle:直肠黏膜组织内源性胱硫醚β合酶和硫化氢表达与溃疡性结肠炎活动性的关系
- Author:
Shuguang ZHU
;
Shuhui LI
;
Peng CHEN
;
Renzhen GUAN
;
Li GAO
- Keywords:
Colitis,ulcerative;
Cystathionine beta-synthase;
Hydrogen sulfide
- From:
Chinese Journal of Postgraduates of Medicine
2016;39(12):1064-1068
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expression of endogenous cystathionine-β-synthase (CBS) and hydrogen sulfide in patients with ulcerative colitis (UC), and explore their possible role in the pathogenesis of UC. Methods Thirty patients with active period UC (active period UC group), 30 patients with remission period UC (remission period UC group) and 30 healthy controls (control group) were selected, and SABC method was used to observe the localization of CBS in rectal mucosal tissues. The optical density value of CBS was analyzed with image analysis systems. The relative expression of CBS mRNA in the rectal mucosa tissue was detected by reverse transcriptase-polymerase chain reaction (RT-PCR), and the expression of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was detected as an internal reference. The expression level of mRNA was detected by semi quantitative analysis with gel imaging analysis system, and the results were expressed as the ratio of the target bands to the GAPDH absorbance. The serum level of hydrogen sulfide was detected. Results The serum level of hydrogen sulfide, optical density value of CBS and relative expression of CBS mRNA of rectal mucosal tissues in active period UC group were significantly higher than those in remission period UC group and control group: (90.13 ± 3.12) μmol/L vs. (50.34 ± 2.34) and (48.13 ± 2.75) μmol/L, 0.433 ± 0.037 vs. 0.295 ± 0.064 and 0.214 ± 0.026, 1.532 ± 0.134 vs. 1.031 ± 0.107 and 0.986 ± 0.067, and there were statistical differences (P < 0.01); but there were no statistical differences between remission period UC group and control group (P>0.05). Conclusions The abnomal expression of CBS and hydrogen sulfide in aactive period UC may play an important role in the pathogenesis of UC.
