Study on antimicrobial resistance and the detection of staphylococcus chromosomal cassette mec typing of methicillin-resistant Staphylococcus aureus in the urban area of Jilin
10.3760/cma.j.jssn.1673-4904.2016.12.007
- VernacularTitle:吉林市区耐甲氧西林金黄色葡萄球菌SCCmec基因分型与耐药性研究
- Author:
Wenyan SHI
;
Xianmin FENG
;
Sheng YU
;
Xin YU
;
Honglan HUANG
- Keywords:
Methicillin- resistant Staphylococcus aureus;
Genotype;
Staphylococcus chromosomal cassette mec typing;
Drug resistance
- From:
Chinese Journal of Postgraduates of Medicine
2016;39(12):1080-1083
- CountryChina
- Language:Chinese
-
Abstract:
Objective To understand the molecular epidemiology characteristics and its drug resistance of methicillin-resistant Staphylococcus aureus (MRSA) in the urban area of Jilin and to provide important basis for guiding the clinical medication and prevention of the MRSA infection. Methods One hundred and three strains of MRSA from July 2013 to July 2014 in the urban area of Jilin were selected. The polymerase chain reaction (PCR) technology and multiple polymerase chain reaction were used to detect mecA gene and Staphylococcus chromosomal cassette mec typing (SCCmec) genotype of MRSA. The drug sensitivity test for 13 kinds of clinical common antibacterial drugs were detected by using the K-B method. And the source of the strains were analyzed. Results The results of SCCmec genotype of MRSA showed that SCCmecⅢtype were 62 strains, accounting for 60.2%;SCCmecⅡtype were 39 strains, accounting for 37.9%; failing to parting were 2 strains,accounting for 1.9%. Drug susceptibility test results showed that all of 103 MRSA strains were resistant to cefoxitin, cefazolin, penicillin and benzene, and drug resistance rate was 100.0%. The resistant rate to erythromycin, levofloxacin, ciprofloxacin, tetracycline, gentamicin and rifampin were 96.1%, 93.2%, 95.1%, 91.3%, 90.3%and 55.3%receptively;the resistant rate to sulfamethoxazolewas was only 1.9%;and the resistant strains to vancomycin and teicoplanin were not detected. The top three department of the distribution of the strains source were department of neurosurgery (31.1%), ICU (19.4%) and burn plastic surgery (17.5%). Conclusions The SCCmecⅢtype is the main MRSA epidemic strains, and SCCmec type II is a minor epidemic strainin the urban area of Jilin. The antibiotic resistance of MRSA is a serious problem with multiple drug resistance, but MRSA is sensitive to vancomycin and teicoplanin.
