Prestin Expression in HEI-OC1 Cells With Oxidative Stress Damage

VernacularTitle:氧化应激损伤与HEI－OC1细胞株中Prestin表达的相关性分析
Author: Junyi WANG ; Xiaomin LAI ; Lijuan GONG ; Yuan XIA ; Zhi WANG
Keywords: Oxidative stress; HEI-OC1 cell; Prestin
From: Journal of Audiology and Speech Pathology 2017;25(1):49-52
CountryChina
Language:Chinese
Abstract: Objective To examine the effects of oxidative stress induced damage to the Prestin expression in HEI-OC1 cells,and to study the mechanism of sensory deafness.Methods We used different concentrations (50μM,100μM,200μM)of hydrogen peroxide canister to cultivate HEI-OC1 cells,and to detect the activity of su-peroxide dismutase(SOD).The quantitative real-time PCR and immunofluorescence were used to detect the prestin expression of mRNA.Results The SOD activity decreased in the HEI-OC1 cells damaged by oxidative stress.The high concentration of the infected group decreased more significantly(F= 9926.293,P<0.01).The expressions of Prestin mRNA and Prestin protein were decreased obviously in the HEI-OC1 cells.The high concentration of in-fected group decreased more significantly (F= 4065.046and7657.217,P<0.01).Conclusion Oxidative stress in-ducing damage inhibits the expression of prestin.Prestin protein may be used as a molecular marker of sensory deafness.