Effects and values of antisurvivin oligonucleotides on IL-6/STAT3 signaling pathway of ovarian cancer cell line
10.7652/jdyxb201701011
- VernacularTitle:Survivin反义寡核苷酸对人卵巢癌细胞株IL-6/STAT3信号通路的影响和意义
- Author:
Yan SUN
;
Chengshu WANG
;
Wenli WANG
- Keywords:
Survivin;
antisurvivin oligonucleotide (ASODN);
ovarian cancer;
metastasis;
IL-6/STAT3 signa-ling pathway;
vascular endothelial growth factor (VEGF)
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2017;38(1):53-57
- CountryChina
- Language:Chinese
-
Abstract:
ABSTRACT:Objective To analyze the effects of antisurvivin oligonucleotide (Survivin ASODN)on IL-6/STAT3 signaling pathway and down-stream cancer genes of ovarian cancer SKOV3 cell line and to detect the changes of invasive ability of cell line in order to explore the role of Survivin ASODN in reducing metastasis and recurrence of ovarian cancer and its potential clinical value.Methods ASODN was transfected into SKOV3 cells by lipofectamineTM2000 in ASODN group,and lipofectamineTM 2000 was introduced in control group.The invasive ability in ASODN and control groups was detected by Transwell chamber.The expressions of interieukin-6 (IL-6 ), signal transducer and activator of transcription 3 (STAT3 ), Survivin, and vascular endothelial growth factor (VEGF)in ovarian cancer cell line at mRNA level were detected by Real-time PCR.The expressions of IL-6 , STAT3 ,signal transducer and activator of transcription 3 phosphorylation (p-STAT3 ),Survivin,and VEGF-A in ovarian cancer cell line at protein level were detected by Western blot.Results The expressions of IL-6,STAT3, Survivin,and VEGF in ovarian cancer cell line at mRNA level were significantly down-regulated in ASODN group (P<0 .0 5 ).IL-6 ,STAT 3 ,p-STAT 3 ,Survivin and VEGF-A protein levels in ovarian cancer cell line were significantly down-regulated in ASODN group (P<0.05).The invasive ability was significantly reduced by ASODN (P<0.01).Conclusion ASODN targeting Survivin could reduce the invasive ability of ovarian cancer cell line. The mechanism may be blocking IL-6/STAT3 signaling pathway and its down-stream cancer genes of ovarian cancer cell line.ASODN targeting Survivin may have clinical value in preventing and treating the metastasis and recurrence of ovarian cancer.
