Content Analysis and Comparative Study of Amino Acids in Ejiao from 18 Manufacturers
10.6039/j.issn.1001-0408.2017.01.32
- VernacularTitle:18家企业阿胶中氨基酸的含量分析与比较研究
- Author:
Chongyu GE
;
Hui PANG
;
Nan LI
;
Jie ZHENG
;
Changlong HE
;
Huan YANG
;
Yuping SHEN
- Keywords:
Ejiao;
Amino acids;
Principal component analysis;
Hierarchical clustering analysis
- From:
China Pharmacy
2017;28(1):122-126
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To provide reference for quality evaluation of Ejiao. METHODS:The contents of 17 amino acids in 18 batches of Ejiao from 18 manufacturers were analyzed by automatic amino acid analyzer;the content data was processed by prin-cipal component analysis (PCA) and hierarchical clustering analysis (HCA),in order to understand the relationship among these amino acids and categorize the Ejiao products. RESULTS:Except for a batch of Ejiao,the other 17 batches contained 17 various amino acids,including 7 necessary amino acids(Thr,Val,Met,Ile,Leu,Phe and Lys)for human being,especially the contents of Gly and Pro were higher. The total content of amino acids was arranged from 66.1% to 82.0%,showing great difference. Four main components were extracted by PCA,the cumulative contribution of which was 89.578%,and accordingly it may be consid-ered that Pro,Ala,Glu,Asp,Leu,Ile and Thr were characteristic amino acids of Ejiao products. In hierarchical clustering analy-sis,3 tree figures of commercial Ejiao product were achieved and Euclidean distance was varied among 0-25. CONCLUSIONS:There is great difference in total control of amino acid in commercial Ejiao product;Ejiao products from various manufacturers dif-fer in their amino acid contents. Ejiao is rich in collagen,and therefore,using amino acid determination would be helpful to moni-tor its quality.
