Expression, purification and characterization of human inhibitory receptor TIGIT in E.coli

VernacularTitle:重组人源抑制型受体TIGIT在大肠埃希菌中的制备及其作用特性研究
Author: Xiaoyun CHEN ; Aiwu ZHOU ; Wei YE
Keywords: TIGIT; clone; expression; purification; Fusobacterium nucleatum; adhesion
From: Journal of Shanghai Jiaotong University(Medical Science) 2017;37(2):199-202
CountryChina
Language:Chinese
Abstract: Objective · To prepare recombinant human TIGIT protein in E.coli and characterize its ability in binding Fusobacterium nucleatum (Fn). Methods · The gene of immunomodulatory protein of human TIGIT was amplified and cloned into pGEX4T2, and recombinant plasmid was transformed into E.coli BL21 (DE3) for GST-TIGIT fusion proteins were purified by the GST affinity chromatography and the interaction between GST-TIGIT fusion protein and Fn was tested by a pulldown assay. Results · Recombinant GST-TIGIT fusion protein expressed successfully in E.coli and was purified to homogeneity by GST affinity column. This protein could specifically bind to Fn, but not Lactobacillus acidophilus. Conclusion · High purify and activity of human GST-TIGIT fusion protein can be achieved by the prokaryotic expression system, and the adhesion between this protein and Fn has been preliminarily explored, which provides basis for further characterize interaction between them.