Simultaneous Determination of Rutin, Hesperidin,Arctiin and Liquiritin in Sangxing Oral Liquid by RP-HPLC
10.6039/j.issn.1001-0408.2017.06.25
- VernacularTitle:RP-HPLC法同时测定桑杏口服液中芦丁、橙皮苷、牛蒡苷和甘草苷的含量
- Author:
Xue CHENG
;
Xuanzhong TAN
;
Lianlin SU
;
Tulin LU
;
Chunqin MAO
;
Xuerong ZHANG
- Keywords:
Sangxing oral liquid;
RP-HPLC;
Rutin;
Hesperidin;
Arctiin;
Liquiritin
- From:
China Pharmacy
2017;28(6):812-815
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for the simultaneous determination of rutin ,hesperidin ,arctiin ,liquiritin and glycyrrhizinate in Sangxing oral liquid. METHODS:RP-HPLC was performed on the column of Kromasil C18 with mobile phase of acetonitrile-0.1% phosphoric acid solution(gradient elution) at a flow rate of 1.0 mL/min,the detection wavelength was 276, 348 nm ,column temperature was 30 ℃,and the injection volume was 5 μL. RESULTS:The linear range was 1.068-10.68 μg/mL (r=0.9993)for rutin,9.320-93.20 μg/mL(r=0.9992)for hesperidin,46.91-469.1 μg/mL(r=0.9994)for arctiin,0.5110-5.110μg/mL(r=0.9992) for liquiritin;RSDs of precision , stability and reproducibility tests were less than 3%;recoveries were 95.10% -101.90%(RSD=2.1%,n=9),96.75%-101.80%(RSD=1.4%,n=9),95.69%-100.00%(RSD=1.5%,n=9), 98.22%-104.60%(RSD=2.1%,n=9),respectively. CONCLUSIONS:The method is accurate,sensitive and reproducible,and can be used for the simultaneous determination of rutin,hesperidin,arctiin and liquiritin in Sangxing oral liquid.