Study on Improving the Quality Standards of Kanggu Zengsheng Tablet
10.6039/j.issn.1001-0408.2017.06.32
- VernacularTitle:抗骨增生片的质量标准提高研究
- Author:
Xi CHEN
;
Linchun WAN
;
Wenting ZHANG
- Keywords:
Kanggu zengsheng tablet;
Naringin;
Icariin;
Acteoside;
Spatholobus suberectus;
TLC;
HPLC
- From:
China Pharmacy
2017;28(6):838-841
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To develop and improve the quality standard for Kanggu zengsheng tablet. METHODS:TLC was used for the qualitative identification of Drynaria fortunei,Epimedii Folium and Spatholobus suberectus;HPLC was used for the contents determination of icariin and acteoside:the column was Diamonsil C18 with mobile phase of acetonitrile-0.1% formic acid solution (gradient elution) at a flow rate of 1.0 mL/min;detection wavelength was 270 nm for icariin and 334 nm for acteoside, Cdumn temperature was 25 ℃,and the injection volume was 10 μL. RESULTS:The TLC spots of D. fortunei,Epimedii Folium and S. suberectus were clear and well separated,negative control without interference. The linear range was 0.0188-1.88 μg for ac-teoside(r=0.9999)and 0.107-2.14 μg for icariin(r=0.9999);RSDs of precision,stability and reproducibility tests were lower than 2.0%;recoveries were 100.2%-105.0%(RSD=1.6%,n=9) and 96.2%-99.5%(RSD=1.4%,n=9). CONCLUSIONS:The improved standard can more effectively control the quality of Kanggu zengsheng tablet.
