Inhibition of Garcinia Acid to Kidney Cancer Cell Lines via Wnt3α/β-catenin Signal Pathway
- VernacularTitle:藤黄酸通过Wnt3α/β-catenin信号通路抑制肾癌细胞的研究
- Author:
Daqing TAN
- Keywords:
Garcinia acid;
Kidney cancer cell lines;
Proliferation;
Apoptosis
- From:
China Pharmacist
2017;20(2):249-252
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the mechanism in growth inhibition of garcinia acid to kidney cancer cell lines ( RC-2 ) . Methods:RC-2 cells were cultured in vitro, and garcinia acid at various concentrations was co-cultured with RC-2 cells. The antipro-liferative activities were determined by CCK-8 assay, the percentage of apoptosis was determined by flow cytometry analysis, and the expression levels of Survivin and related proteins in Wnt3α/β-catenin signal pathway were measured using Western Blot analysis. Re-sults:After the treatment with garcinia acid for 24h and 48h, the proliferation of RC-2 cells was significantly suppressed by garcinia acid in a dose-dependent manner, and all garcinia acid groups had significantly higher apoptosis percentage of RC-2 cells than the con-trol group. In G0/G1 period, RC-2 cells reduced while increased in G2/M period, and in S period, the cells remained the same a-mount. The expression levels of Survivin, Wnt3α and β-catenin decreased in RC-2 cells after the treatment with garcinia acid. Con-clusion:Garcinia acid can inhibit the proliferation and promote the apoptosis of RC-2 cells, and the mechanism may be related with the inhibition of Wnt3α/β-catenin signal pathway and further decreasing the expression levels of Survivin.
