Immunoregulatory effect of Buyang-Huanwu decoction on monocyte-mac-rophages in mice with experimental autoimmune encephalomyelitis
10.3969/j.issn.1000-4718.2017.02.002
- VernacularTitle:补阳还五汤对实验性自身免疫性脑脊髓炎单核巨噬细胞的免疫调控作用
- Author:
Qianqian TIAN
;
Yanhua LI
;
Jiezhong YU
;
Xiaoqing WANG
;
Hongzhen ZHANG
;
Jianchun LIU
;
Wenjuan GUO
;
Chunyun LIU
;
Chunyan YANG
;
Baoguo XIAO
;
Cungen MA
- Keywords:
Buyang-Huanwu decoction;
Experimental autoimmune encephalomyelitis;
Macrophage
- From:
Chinese Journal of Pathophysiology
2017;33(2):200-207
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To explore the therapeutic effect of Buyang-Huanwu decoction (BYHWD) on experimental au-toimmune encephalomyelitis ( EAE) and its immunoregulatory effect on monocyte-macrophages .METHODS: Chronic EAE was induced by myelin oligodendrocyte glycoprotein peptide fragment 35-55 ( MOG35-55 ) in the female C57BL/6 mice, which were randomly divided into saline group and BYHWD group .On day 3 after immunization , the mice in BYHWD group were orally administrated with BYHWD , while normal saline was given to the control mice .The clinical score and body mass were recorded every other day .At day 17 after immunization , the mice were sacrificed and spinal cords were obtained for HE staining and myelin staining .The M1 and M2 macrophage phenotypes of splenic cells were detected by flow cytometry and immunofluorescence staining .The protein expression of iNOS , TNF-α, arginase and IL-10 in the spinal cord macro-phages was determined by Western blotting .RESULTS:BYHWD delayed the onset of EAE , reduced the clinical scores of EAE, inhibited the inflammatory cell infiltration and demyelination in the spinal cord , and promoted the conversion of M 1 macrophages into M2 phenotype in the spinal cord and spleen .CONCLUSION:BYHWD intervention attenuates the be-havioral and pathological changes in the EAE mice , and its mechanism may be related to the macrophage conversion .
