Roscovitine inhibits inflammatory hyperplasia of carotid artery intima in rats via suppressing nuclear factor-κB activation
10.3969/j.issn.1000-4718.2017.02.007
- VernacularTitle:Roscovitine 通过影响核因子κB 活化抑制大鼠颈动脉内膜损伤导致的炎性增生
- Author:
Jingshan ZHAO
;
Jiahuan SUN
;
Kun YU
;
Yu LIU
;
Chao WANG
;
Aiying LI
- Keywords:
Roscovitine;
Nuclear factor-κB;
Carotid artery intima;
Inflammatory hyperplasia
- From:
Chinese Journal of Pathophysiology
2017;33(2):233-238
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To study the effect of roscovitine on the inflammatory hyperplasia of carotid artery intima in rats and the related mechanisms .METHODS: SD rats ( n=60 ) were randomly divided into 3 groups including control group, model group and treatment group .The rat model was established by trypsin digestion injury .The rats in control group were given sham operation .The rats in treatment group were administered with 0.5 mL roscovitine (2 g/L) slow-re-leasing gelatin.The rats in each group were fed normally for 4 weeks, then killed to take out carotid arteries for further ob-servations .The effects of roscovitine on the inflammatory hyperplasia of carotid artery intima and the related mechanism via nuclear factor-κB ( NF-κB) in the rats were detected by Western blot .RESUITS:Roscovitine inhibited the activation of NF-κB and the expression of inflammatory factors cyclooxygenase-2 (COX-2), vascular cell adhesion molecule-1 (VCAM-1),TNF-αand IL-6 via blocking the phosphorylation activation of NF-κB and inhibiting the degradation of IκB-α.CON-CLUSION:Roscovitine inhibits inflammatory hyperplasia of carotid artery intima in the rats via suppressing NF-κB activa-tion.
