Effect of low expression of miR-21 on apoptosis of HepG2 cells induced by matrine
10.3969/j.issn.1000-4718.2017.02.015
- VernacularTitle:低表达miR-21对苦参碱诱导的肝癌HepG2细胞凋亡的影响
- Author:
Yaoling LUO
;
Youxin HUANG
;
Ping LAI
;
Minhong ZHANG
- Keywords:
miR-21;
Matrine;
HepG2 cells;
Apoptosis
- From:
Chinese Journal of Pathophysiology
2017;33(2):284-288
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To explore whether miR-21 low expression enhances the effect of matrine ( MAT) on the ap-optosis of hepatocellular carcinoma cells .METHODS:Real-time fluorescence quantitative PCR ( RT-qPCR) was used to detect the expression of miR-21 in the HepG2 cells treated with different concentrations of MAT .The effect of miR-21 on MAT-induced HepG2 cell apoptosis was analyzed by flow cytometry .The mRNA and protein expression of Bcl-2 and Bax in the HepG2 cells treated with MAT was determined by RT-qPCR and Western blot .RESULTS:The expression of miR-21 increased with the increasing concentration of MAT .Low expression of miR-21 promoted MAT-induced apoptosis , and en-hanced the expression of Bax at mRNA and protein levels ( P<0.05 ) , while inhibited the expression of Bcl-2 at mRNA and protein levels (P<0.05).CONCLUSION:Low expression of miR-21 enhances MAT-induced HepG2 cell apoptosis by inhibiting the expression of Bcl-2 and promoting Bax expression .
