Differential regulation of SREBP 1 c/cm on transcriptional activity and expression of PERK promoter
- VernacularTitle:SREBP1 c／cm对PERK启动子转录活性及表达的差异性调控
- Author:
Qin HU
;
Yu MAO
;
Jialing LU
;
Weiwei XIE
;
Wei ZHENG
;
Fengjin GUO
- Keywords:
PERK;
SREBP1c;
transcriptional activity;
lipid metabolism and ERs
- From:
Basic & Clinical Medicine
2017;37(2):162-168
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of sterol regulating element binding protein (SREBP1c) and its ac-tive form (SREBP1cm) on human protein kinase R-like endoplasmic reticulum kinase (PERK).Methods Re-porter victors of PERK promoter and its truncations were constructed with pGL 3-basic and co-transfected with internal reference pRL-SV40 into cell and luciferase activity was detected .pcDNA3.1 ( -)-SREBP1c or pcDNA3.1 ( -)-SREBP1cm was co-transfected with PERK promoter transcriptional activity core regions and the detection of dual -lu-ciferase reporter gene was used to analyze the regulation of SREBP 1c/1cm on PERK promoter transcriptional activity . The expression level of PERK mRNA and protein were detected by RT-PCR and Western blot .Results PERK pro-moter and truncations were successfully constructed into pGL 3-basic, and PERK promoter core area of transcription-al activity had determined;Dual-luciferase report gene showed that SREBP 1c inhibited PERK promoter transcrip-tional activity and SREBP1cm promoted PERK promoter transcriptional activity .RT-PCR and Western blot showed that SREBP1c decreased PERK mRNA and protein expression , but SREBP1cm increased PERK mRNA and protein expression, which was consistent with the detection of dual-luciferase report gene .Conclusions SREBP1c and SREBP1cm have a opposite regulation effect on PERK promoter transcriptional activity and its expression .
