Application of droplet digital PCR in the precision treatment of breast cancer
10.3969/j.issn.1000-8179.2017.03.300
- VernacularTitle:液滴数字PCR在乳腺癌精准治疗中的应用
- Author:
Bocheng ZHANG
;
Xianyu ZHANG
;
Gen SUN
;
Da PANG
- Keywords:
droplet digital PCR;
breast cancer;
precision treatment;
gene mutation;
gene detection
- From:
Chinese Journal of Clinical Oncology
2017;44(3):146-149
- CountryChina
- Language:Chinese
-
Abstract:
In the context of precision medicine, although individual treatment of breast cancer under the guidance of molecular classi-fication has become the norm, a precision treatment program with increased efficiency and quality is still required. Compared with the traditional real-time fluorescent quantitative polymerase chain reaction (PCR), the droplet digital PCR (ddPCR) has obvious advantages in the detection of rare mutations and copy number variations, as well as the integration with the second-generation-sequencing tech-nology. This paper reviews the application of a ddPCR platform in different breast cancer subtypes and explores new horizons of breast cancer research through the ddPCR technology.
