MiR-200c/141 methylation inhibits the expression of miR-200c and miR-141 in gastric cancer
10.3969/j.issn.1000-8179.2017.02.119
- VernacularTitle:胃癌中DNA甲基化对miR-200c 141表达影响的研究
- Author:
Xinliang ZHOU
;
Cong ZHANG
;
Yudong WANG
;
Lianmei ZHAO
;
Meixiang SANG
;
Baoen SHAN
- Keywords:
gastric cancer;
methylation;
miR-200c;
miR-141;
clinicopathological feature
- From:
Chinese Journal of Clinical Oncology
2017;44(2):73-77
- CountryChina
- Language:Chinese
-
Abstract:
Objective:This work aims to detect the levels of miR-200c/141 methylation and miR-200c/141 in gastric cancer tissue and investigate the relationship between miR-200c/141 expression and clinical parameters. Methods:The methylation status of miR-200c/141 CpG island and miR-200c/141 in gastric cancer tissue specimens was evaluated by qRT-PCR or BS-MSP method. We analyzed the relationship among the methylation status of miR-200c/141 CpG island, expression level of miR-200c or miR-141, and clinical parame-ters. Results:The status of miR-200c/141 CpG island methylation in gastric cancer tissue was significantly higher compared with that in paracarcinoma tissue. MiR-200c and miR-141 were markedly decreased in gastric cancer tissue compared with those in adjacent tis-sue. MiR-200c/141 CpG island methylation was negatively related with the expression of miR-200c and miR-141 in gastric cancer speci-mens. Conclusion:The upregulation of miR-200c/141 CpG methylation inhibits miR-200c/141 expression in gastric cancer tissue.
