Study on Quality Standard of Sanyuan Rupixiao Gel Paste
10.3969/j.issn.1005-5304.2017.03.019
- VernacularTitle:三元乳癖消凝胶膏质量标准研究
- Author:
Zhuo WANG
;
Yuchuan CHENG
;
Yuanyuan LI
;
Dingding GUO
;
Yan NI
;
Xuliang HAO
;
Peng KONG
;
Jiaoni YAO
;
Ze LIANG
- Keywords:
Sanyuan Rupixiao Gel Paste;
Impatientis Semen;
quality standard;
TLC;
HPLC
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2017;24(3):78-81
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the quality standard for Sanyuan Rupixiao Gel Paste. Methods Sparganii Rhizoma, Gleditsiae Sinensis Fructus, Cyperi Rhizoma and Impatientis Semen were identified by TLC method. The content of tetrahydropalmatine was determined by HPLC. Waters symmetry column was used with the mobile phase of acetonitrile-0.1% phosphatic acid in a gradient manner (pH was adjusted to 6.4 by triethylamine) (55:45) at the detection wavelength of 280 nm. The flow rate was 1.0 mL/min at the column temperature of 30 ℃. Results The spots in TLC were clear without any interference;tetrahydropalmatine showed a good linear relation in the range of 0.092–1.84 μg;the average recovery was 100.15%with RSD of 1.58%(n=6). Conclusion The method is simple and accurate with high reproducibility, which can be used for the quality control of Sanyuan Rupixiao Gel Paste.
