The effect and mechanism of resveratrol on autophagy regulation in nasopharyngeal carcinoma CNE-2 cells
10.11958/20160975
- VernacularTitle:白黎芦醇对鼻咽癌CNE-2细胞自噬的影响及机制
- Author:
Min WANG
;
Hong ZHAO
;
Jiafa ZHENG
;
Yuanjie XIE
;
Pingbo YAO
- Keywords:
nasopharyngeal neoplasms;
autophagy;
Resveratrol;
CNE-2
- From:
Tianjin Medical Journal
2017;45(2):143-145
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of resveratrol on autophagic flux of nasopharyngeal carcinoma CNE-2 cells, and to explore the underlying mechanism. Methods Nasopharyngeal carcinoma CNE-2 cells were divided into control group and resveratrol group. Cells in control group were normally cultured at 37℃and received no further treatment. Resveratrol group was added 40 μmol/L resveratrol 2 h before cells were culture at 37 ℃. Western blot analysis was performed to detect protein expressions of LC3B, p62, Beclin-1, phospho-mTOR (p-mTOR) and phospho-S6 (p-S6). The autophagic flux was detected under the confocal laser scanning microscopy through different color spots, after cells were transfected with adenovirus encoding GFP-mRFP-LC3. Results (1) The protein expression of LC3B was significantly increased and the protein expression of p62 was significantly decreased in resveratrol group compared with those of control group (P<0.05). There was no significant difference in Beclin-1 expression between two groups. (2) Compared to control group, expressions of p-mTOR and p-S6 were significantly decreased in resveratrol group (P<0.05). (3) Compared to control group, the red mRFP puncta were significantly increased, and the yellow GFP puncta were significantly decreased in resveratrol group (P<0.05). Conclusion Resveratrol promotes the autophagic flux of nasopharyngeal carcinoma CNE-2 cells, and the effects are possibly dependent on the activation of mTOR pathway-related proteins.
