Mir-483 enhances the proliferation,invasion and migration of nasopharyngeal carcinoma cells
10.3969/j.issn.1006-5725.2017.02.003
- VernacularTitle:Mir-483增加鼻咽癌细胞的生长、侵袭和迁移能力
- Author:
Miaohong YAN
;
Shasha DU
;
Rong LI
;
Jie LIN
;
Tan CHEN
;
Yawei YUAN
- Keywords:
Nasopharyngeal carcinoma;
MicroRNA;
Mir-483
- From:
The Journal of Practical Medicine
2017;33(2):177-180
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of mir?483 on the proliferation,invasion and migration of nasopharyngeal carcinoma cells . Methods RT?qPCR was used to detect mir?483 expression level in different nasopharyngeal carcinoma cells(CNE?1,CNE?2)and the alteration of mir?483 expression level in these cells after radiation. CNE?1,one of the NPC cells,was selected. Cationic liposomes transient transfection was used to construct cells which overexpressed mir?483 or negative control . MTT assay was conducted to test the cell proliferation ability. Wound scratch assay and transwell migration assay were used to detect the effects of mir?483 on NPC invasion and migration ability. Results In terms of radiation resistance ,the expression level of mir?483 was higher in CNE?1. Overexpressing mir?483 can enhance the proliferation ,invasion and migration ability of CNE?1. Conclusions Mir?483 enhances the proliferation,invasion and migration ability of nasopharyngeal carcinoma cells.
