The effect of chemotherapeutic drugs on CD19-CAR-T cells in vitro
10.3969/j.issn.1006-5725.2017.02.009
- VernacularTitle:化疗药物对CD19嵌合抗原受体T细胞的体外抑制作用
- Author:
Wenfang YI
;
Mo YANG
;
Zhiyong PENG
;
Yuelin HE
;
Huayin LIU
;
Chunfu LI
- Keywords:
CD19-CAR-T cell;
Fludarabine;
Mafosfamide;
Busulfan;
Cyclophosphamide
- From:
The Journal of Practical Medicine
2017;33(2):198-201
- CountryChina
- Language:Chinese
-
Abstract:
Objective The research about the effect of different chemotherapeutic drugs on CD19?CAR?T cells with CCK8 test to provide the theoretical support about the development of chemotherapy for clinical support. Methods Extract T cells from a normal adult peripheral blood and synthesize CD19?CAR?T cell. CD19?CAR?T cells were treated with different doses of chemotherapeutic drugs for 24,48,72 h and(or)96 h,and inhibition rate was calculated. Results First,we observed that the inhibition rates of fludarabine and Mafosfamide for CD19?CAR?T cells were increasing with the time and concentration (P < 0.05). Secondly , Bus ulfan had no effect in CD19?CAR?T cells in vitro(P > 0.05). Finally ,Cyclophosphamide had no effect in CD19?CAR?T cells in vitro (P > 0.05). Conclusion Mafosfamide and Fludarabine can inhibit the CD19?CAR?T cells. Cyclophosphamide have no activity in vitro.
