Effect of Polymorphisms of the Methylenetetrahydrofolate Reductase Gene (C677T,A1298C and G1793A)on Stillbirth
10.3969/j.issn.1671-7414.2017.01.020
- VernacularTitle:MTHFR（C677T，A1298C和G1793A）基因多态性对死胎的影响
- Author:
Juanjuan HUANG
;
Hongyun YUE
;
Juan ZHANG
- Keywords:
stillbirth;
MTHFR;
twin pregnancy
- From:
Journal of Modern Laboratory Medicine
2017;32(1):72-76
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the relationship between Methylenetetrahydrofolate reductase (MTHFR)(C677T, A1298C and G1793A)polymorphisms and stillbirth.Methods 33 unexplained stillbirth and 582 normal livebirth were col-lected for case-control study from August 2014 to August 2016.The genotyping of C677T,A1298C and G1793A polymor-phisms of the MTHFR gene was tested by using restriction fragment length polymorphism polymerase chain reaction.The relationship between MTHFR polymorphisms and stillbirth susceltibility was analyzed by logistic regression.Results There was no significant difference in the genetype frequencies of A1298C and G1793A of MTHFR polymorphisms between case and control.But there was significant difference in the genetype frequencies of C677T between case and control (CT:P=0.02,χ2=3.67;TT:P=0.02,χ2=3.65).Heterozygous CT and homozygous TT would be high risk factors of stillbirth. C677T was found in one (14.3%)of the cases with early stillbirth and in three (11.5%)of the cases with late stillbirth, having no significant difference.Twin pregnancy and MTHFR C677T increased the risk 8-fold (P<0.001,χ2=13.28)and 3.4(P=0.02,χ2=3.65)for stillbirth,respectively.Conclusion MTHFR C677T polymorphism is high risk factors associ-ated with the susceptibility of stillbirth.MTHFR C677T may be used as a prognostic marker of pregnancy cases predisposed to preterm delivery.
