Evaluation of the Nucleated Red Blood Cells Count of Sysmex XN-9000 Automatic Hematology Analyzer and the Validation of Manual Method

VernacularTitle:XN-9000全自动血液细胞分析仪有核红细胞计数与手工方法验证的探讨
Author: Qin LI ; Jia SHI ; Xingbing LU ; Zhigang MAO ; Xinqin ZHU ; Hong JIANG ; Sugen ZENG
Keywords: automatic hematology analysis line; NRBC count; manual validation method
From: Journal of Modern Laboratory Medicine 2017;32(1):124-127
CountryChina
Language:Chinese
Abstract: Objective To evaluate the nucleated red blood cell (NRBC)count of Sysmex XN-9000 automatic hematology anal-ysis lines comparing with manual method,and verify the accuracy of the analyzer results.Methods 60 blood samples with more than 1% of NRBCs detected by XN-9000 were counted NRBCs by traditional manual microscopy in blood smears,and verified the analyzer results.Results According to the reliability analysis,the results of total 60 samples were all within the range of the reliability;correlation analysis showed that correlation coefficient (r)of group NRBC (%)1~10 and>10 were 0.972 1 and 0.996 2,respectively.There were no significant differences between them (P>0.05).Conclusion Compared with manual method,the results of NRBC count of XN-9000 were within the range of reliability,and showed good correla-tion.The analyzer test results of NRBC were accurate and reliable and could be applied to the detection of clinical samples.