The development and prospects of high-resolution melting analysis
10.3760/cma.j.issn.1009-9158.2017.02.001
- VernacularTitle:高分辨熔解曲线分析技术的发展与展望
- Author:
Zhaojing ZHENG
;
Qihua FU
;
Luming ZHOU
- Keywords:
Polymerase chain reaction;
Fluorescent dyes;
Molecular diagnostic techniques;
High resolution melting;
Algorithms
- From:
Chinese Journal of Laboratory Medicine
2017;40(2):77-79
- CountryChina
- Language:Chinese
-
Abstract:
High-resolution melting analysis ( HRMA/HRM ), a simple, rapid, flexible, inexpensive closed tube approach with high sensitivity and specificity has been one of the most widely used molecular diagnostic techniques in clinicalas well as in research settings .Recently, rapid development ofinstruments , DNA dyes and analysis software significantly enhance the sensitivity , specificity and accuracy of HRM,providing a fast, efficient and economic molecular diagnostic platform for molecular diagnosis of inherited disease , molecular profiling and target therapy of cancer , identification of pathogen , as well as individualized medicine.
