Clinical application of high resolution melting curve assay in bacteria identification and antimicrobial susceptibility testing
10.3760/cma.j.issn.1009-9158.2017.02.003
- VernacularTitle:高分辨熔解曲线分析在临床细菌鉴定及药敏检测中的应用
- Author:
Aiping ZHOU
;
Wenjuan WU
- Keywords:
Bacterial infections;
Drug resistance,bacterial;
Microbial sensitivity tests;
High resolution melting
- From:
Chinese Journal of Laboratory Medicine
2017;40(2):84-87
- CountryChina
- Language:Chinese
-
Abstract:
High resolution melting ( HRM) analysis is a real-time PCR based technique which can detect DNA sequence polymorphism .Immediately after routine PCR with saturated fluorescent dye , melting transition of the amplicon was monitored and fluorescence was collected .According to the different melting curve, single base differences can be distinguished .HRM is a low -cost, single -step, closed -tube, accurate and rapid method , which has aroused general concern .Rapid and sensitive detection of the clinical pathogens is critical to diagnosis of infectious diseases .However , clinical research on application of HRM in infectious disease pathogen identification and drug resistance , especially melting curve analysis based bedside technology integration will provide strong support for the diagnosis and treatment of infectious diseases.
