Prediction and analysis of T-cell and B-cell epitopes of Mycobacterium tuberculosis Rv2657c protein
10.3969/j.issn.1006-5725.2017.01.015
- VernacularTitle:结核潜伏感染蛋白Rv2657c T细胞和B细胞表位的预测与分析
- Author:
Dan YANG
;
Xuejuan BAI
;
Yourong YANG
;
Minggui LIN
;
Xueqiong WU
- Keywords:
Tuberculosis;
Rv2657c;
Epitope prediction
- From:
The Journal of Practical Medicine
2017;33(1):55-58
- CountryChina
- Language:Chinese
-
Abstract:
Objective To predict the epitopes of Mycobacterium tuberculosis Rv2657c protein, to understand its immunogenicity Methods The T?cell and B?cell epitopes of Mycobacterium tuberculosis Rv2657c protein were predicted by DNAStar software package. The homology of Rv2657c amino acid sequence with the human protein sequences was prepared using Blast method, then the CTL epitopes were predicted using SYFPEITHI supermotif method, BIMAS quantitative motif method and NetCTL prediction method, and the Th epitopes were predicted by RANKPEP and SYFPEITHI supermotif prediction method. Results The prediction using DNAStar software package showed that Rv2657c protein had 5 B?cell epitopes and 6 T?cell epitopes. The protein had 6 CTL epitopes and there were 38 Th epitopes. Conclusion Rv2657c protein has both B?cell epitopes and T?cell epitopes. It may be a candidate target antigen for the studies of vaccine and diagnosis of tuberculosis.
