Role and bioinformatics analysis of UCP2 in cervical cancer

VernacularTitle:线粒体解耦联蛋白2在宫颈癌中的作用及生物信息学分析
Author: Jing JIANG ; Yan CHEN ; Hong WU
Keywords: Cervical cancer; Uncoupling protein 2; Bioinformatics
From: The Journal of Practical Medicine 2017;33(1):91-94
CountryChina
Language:Chinese
Abstract: Objective To investigate the effect of UCP2 on cell proliferation, apoptosis, metastasis in cervical squamous carcinoma. Methods Plasmid?mediated downregulation of UCP2 was obtained in cervical cancer cell lines. MTT, flow cytometry and transwell chamber assay were conducted to detect the ability of proliferation, apoptosis and metastasis. Characteristics of UCP2 protein was analyzed by bioinformatics methods. Results (1)UCP2 was verified to be downregulated by qRT?PCR and Western blotting assay.(2)MTT, apoptosis assay and transwell chamber assay showed that the proliferation of SiHa cell was significantly inhibited, and the apoptosis rate was significantly increased, and metastasis was markedly deduced (P < 0.05). (3) Bioinformatic analysis showed that UCP2 was located in mitochondria with several phosphorylation sites, and UCP2 interacted with other proteins to produce biological effects. Conclusion UCP2 may play an important role in the occurrence and development of cervical cancer, and it is expected to be a new target for the early diagnosis and treatment of cervical cancer.