Detection and the Significance of SOCS-1 Gene Methylation Status in Peripheral Blood of Systemic Lupus Erythematosus Patients
10.3969/j.issn.1671-7414.2017.01.010
- VernacularTitle:系统性红斑狼疮患者外周血SOCS-1基因甲基化的检测及意义
- Author:
Hai DING
;
Shuo GAO
;
Hongxia WEI
;
Lei LI
;
Qingfei WANG
- Keywords:
systemic lupus erythematosus;
SOCS-1;
gene methylation
- From:
Journal of Modern Laboratory Medicine
2017;32(1):34-37
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationship between the systemic lupus erythematosus (SLE)and the SOCS-1 gene methylation status of the peripheral blood DNA,to provide the basis for diagnosis and treatment of systemic lupus erythema-tosus.Methods Blood samples of SLE patients (27 cases)and healthy group (19 cases)in January 2015 to April were col-lected and the DNA were extracted.Using polymerase chain reaction combining DNA agarose gel electrophoresis to detect the SOCS-1 gene methylation status.Results In patients with systemic lupus erythematosus SOCS-1 gene complete methyl-ation accounted for 44% (12/27),incomplete methylation accounted for 56% (15/27).In healthy group SOCS-1 gene com-plete methylation accounted for 74% (14/19)and incomplete methylation accounted for 26% (5/19).The rate of complete methylation of SOCS-1 gene of SLE patients was lower than that of healthy group (χ2=3.88,P=0.049).Conclusion SLE patients may have lower SOCS-1 gene methylation status in the peripheral blood DNA,which is worth for further study.
