Development and effectiveness evaluation of time-resolved fluoroimmunoas-say kit for detection of Schistosoma japonicum
10.16250/j.32.1374.2016157
- VernacularTitle:血吸虫病时间分辨免疫荧光检测试剂盒的研制和效能评价
- Author:
Qingdong MENG
;
Yan WANG
;
Wenqiao SUN
;
Shoulei REN
;
Bo XIN
;
Pengfei ZHU
;
Kangyan LI
;
Huankun LIANG
;
Licheng ZHANG
- Keywords:
Schistosoma japonicum;
Recombinant protein SjP38;
Double-antibody sandwich;
Time-resolved fluoroimmuno-assay(TRFIA)
- From:
Chinese Journal of Schistosomiasis Control
2017;29(1):64-67
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a kit of time?resolved fluoroimmunoassay(TRFIA)for detection of Schistosoma japonicum protein SjP38,and evaluate its effectiveness. Methods The anti 9G7 SjP38 monoclonal antibody was used as the capture anti?body coated with 96?hole plate,and the Eu3+labeled 1A6 monoclonal antibody was used as the detection antibody to establish the TRFIA SjP38 kit. In addition,the accuracy,sensitivity,precision,stability and coincidence rate to pathogenic diagnosis of the kit were evaluated. Results This established kit possessed high accuracy,wide linear range from 2 to 1 250 ng/ml,high sensitivity with the minimum detectable concentration of 0.14 ng/ml,and good precision(the coefficient variation of the intra?and inter?assay were 3.6%to 4.6%and 5.1%to 6.7%,respectively). The stability tests showed that the reagents could be stable for six months at 4℃,7 d at 37℃. The positive and negative corresponding rates to the pathogen detection method were 95%and 100%respectively. Conclusion All the performance and detection indicators of the kit have reached the requirements of clinical test,but its clinical application still needs further validation.
