Effect of lipoxin A4 on lipopolysaccharide-induced oxidant stress in human renal tubular epithelial cells and possible mechanisms

VernacularTitle:脂氧素对脂多糖诱导的人近曲肾小管上皮细胞氧化应激的影响及机制
Author: Fen XU ; Hua HAO ; Hua DAI ; Lixiang LI ; Lei ZENG
Keywords: Lipoxin; Nrf2; Oxidant stress
From: The Journal of Practical Medicine 2017;33(1):51-55
CountryChina
Language:Chinese
Abstract: Objective The study aimed to investigate the effect of lipoxin A4 (LXA4) on lipopolysaccharide (LPS)?induced oxidant stress in human renal tubular epithelial cells (HK2 cells) and possible underlying mecha?nisms. MethodsHK2 cells were divided into three groups: Control ,LPS and LPS+LXA4 groups. After cells were treated with indicated conditions,morphological changes were observed. The expressions of Nrf2 were detected by immunofluorescence and cells were collected for RT?PCR experiments.Results HK2 cells seemed disrupted and necrotic with the administration of LPS. However ,LXA4 could prevent cells from injury induced by LPS. LPS decreased Nrf2 expression and promoted it to translocate to cytoplasm ,while LXA4 could increase its expression and promote it to translocate to nucleus. Moreover ,LPS could decrease Nrf2 and its downstream molecule mRNA expressions,but LXA4 could reverse this effect. Conclusion Our results demonstrated that LXA4 effectively inhibit?ed HK2 cell oxidant stress via Nrf2 pathway.