MicroRNA expressions in peripheral blood plasma of the residents from high background radiation area of Yangjiang, China
10.3760/cma.j.issn.0254-5098.2017.01.001
- VernacularTitle:阳江高本底地区居民外周血血浆miRNAs 的表达
- Author:
Pinhua ZHANG
;
Gang GAO
;
Yan PAN
;
Mei TIAN
;
Lina WU
;
Chunnan PIAO
;
Jianlei RUAN
;
Jianxiang LIU
- Keywords:
High background;
Low dose radiation;
miRNAs;
Plasma;
Biomarker
- From:
Chinese Journal of Radiological Medicine and Protection
2017;37(1):1-6
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of high background radiation on the expressions of miR-16, miR-106b, miR-449a, miR-34a and let-7g in peripheral blood plasma of the residents .Methods Totally 110 healthy female long-term local residents aged over 50 years were randomly selected from the high background radiation area and the control area , while their age, body mass index(BMI) and other indicators were surveyed .The relative expression levels of miRNAs in peripheral blood plasma of these women were quantitatively detected by real-time fluorescence quantitative PCR ( RT-PCR) .Then t-test was used to analyze the cumulative dose , age and BMI between the high background and control group .Mann-Whitney U-test was used for statistical analysis of miRNA expression levels between two groups , and the multiple regression analysis was used finally .Results Compared with the control group , the cumulative dose of individuals in the high background group was about four times higher (t=42.803, P<0.05), and the levels of miR-16 and miR-106b in plasma of high background group were down-regulated, while the level of miR-449a was up-regulated ( Z =4.180, 2.422, 2.794, P <0.05 ).After controlling of confounding factors such as age and BMI , the expression levels of miR-16 and miR-106b were negatively correlated with the cumulative dose of individuals (P<0.05).On the contrary, no significant correlation was observed between the levels of miR-449a, miR-34a, let-7g and the individual cumulative dose (P>0.05).Conclusions miR-16 and miR-106b may serve as biomarkers for the early stage of low dose radiation health effects .
