Detection of hepatitis B virus protein and gene in intrahepatic and extrahepatic cholangiocarcinoma tissue
10.11904/j.issn.1002-3070.2017.01.002
- VernacularTitle:乙肝病毒蛋白和基因在肝内和肝外胆管癌组织中的检测及意义
- Author:
Yiqi WU
;
Ying LUI
;
Li ZHAO
;
Xiaoming JIN
- Keywords:
Cholangiocarcinoma;
Hepatitis B virus;
Intrahepatic cholangiocarcinoma;
Extrahepatic cholangiocarcinoma
- From:
Practical Oncology Journal
2017;31(1):7-12
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the importance of hepatitis B virus ( HBV ) infection in the patho-genesis of cholangiocarcinoma ( CC) and further clarify the correlation between the occurrence of intrahepatic and extrahepatic cholangiocarcinoma .Methods HBV protein and gene of 52 formalin fixed,paraffin embedded tis-sues with CC were detected by immunohistochemistry and nest PCR .Results Hepatitis B virus X gene was de-tectable in 33.3%(7/21)of 21 intrahepatic cholangiocarcinoma cases .Hepatitis B surface antigen(HBsAg)was detectable in 21.7%(5/21)and hepatitis B core antigen(HBcAg)was detectable in 19.0%(4/21)of 21 intrahe-patic cholangiocarcinoma cases .In contrast,no HBsAg,HBcAg and hepatitis B virus X gene were detected in any of the 31 extrahepatic cholangiocarcinoma cases .Conclusion HBV infection is a significant risk factor for intra-hepatic cholangiocarcinoma ,but not for extrahepatic cholangiocarcinoma ,in Northeast of China .The integration of HBV gene may be involved in the carcinogenesis of intrahepatic bile duct epithelial cells .
