Investigation into the isobaric tags for relative and absolute quantitation-based serum biomarkers screening in primary gout patients

VernacularTitle:基于同位素标记相对和绝对定量技术筛选原发性痛风的血清生物标记物研究
Author: Yong CHEN ; Ying YING ; Haiyan HUANG ; Zhen ZHANG ; Xiudi WU ; Xianqian HUANG ; Yong PENG ; Minzhi GAN ; Baoqing GENG ; Mengya ZHU
Keywords: Gout; Isotope labeling; Biological markers
From: Chinese Journal of Rheumatology 2017;21(1):4-9
CountryChina
Language:Chinese
Abstract: Objective To identify primary gout biomarkers. Methods Isobaric tags for relative and absolute quantitation (iTRAQ) technique combined with liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to screen differentially expressed proteins, and to identify potential biomarkers by analysis of the biological process, cellular components, molecular functions, KEGG pathways and protein-protein interactions. Difference between two groups were measured byt test. Results We identified 95 differentially expressed proteins (50 up-regulated proteins and 45 down-regulated proteins, respectively), and 20 significant KEGG pathways. Among them, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), α-enolase (ENOA), phosphoglycerate kinase (PGK1), glucose-6-phosphate isomerase (G6PI) and moesin might play a role in the pathogenesis of primary gout. Conclusion iTRAQ technology can detect differentially expressed proteins from proteome, provides a strong theoretical basis for the study of biomarkers and evidence for the mechanisms in primary gout. However, further studies are needed.