Expression of miR-134 in Methamphetamine-induced Neuronal Injury and Its Effect on Evoked Action Potential
- VernacularTitle:MMiR-134在MA诱导神经元损伤中的表达及其对神经诱发动作电位的影响
- Author:
Tao LI
;
Hongjie WANG
;
Guiyang LIU
;
Bo ZHANG
;
Wenbo ZHAO
;
Tianwei LIU
;
Kaiwen ZHU
;
Jinhao SUN
- Keywords:
miR-134;
methamphetamine;
neurotoxicity;
action potential
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2017;38(1):36-41
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To investigate the expression change of miR-134 in methamphetamine(MA)-induced neuronal injury in PC12 cells and its effect on neuronal excitability and understand the pathogenesis of methamphetamine-induced neuronal injury.[Methods]PC12 cells in the logarithmic phase were divided into control group and MA group. The MA group was treated with 800μmol/L MA to establish the model of neuronal injury. The cellular injury was observed under microscope. The neuronal apoptosis was detected by Hoechst3342/PI double staining,and miR-134 expression was measured by using real-time quantitative PCR (Real time-PCR). Furthermore,we constructed miR-134 interference vector and observed its effect on evoked action potential.[Results]The cultured PC12 cells were damaged under the 800 μmol/LMA treatment ,and neurites became shorter ,the apoptotic cells were evidence. Real time-PCR showed that miR-134 expression was increased after MA treatment. Electrophysiological data showed that the evoked action potential increased after miR-134 interference.[Conclusions]High concentration of MA can induce neuronal damage and apoptosis and also increase miR-134 expression. While silence miR-134 expression can increase neuronal excitability.Our study provides an experimental basis for elucidating the possible mechanism of MA-induced neuronal injury and the role of miR-134 in neurotoxicity and neuronal excitability.
