Effects of caveolin-1 on expressions of interleukin-1βand interleukin-6 in the ischemic cortex after permanent middle cerebral artery occlusion in mice
10.3760/cma.j.issn.1673-4165.2016.11.008
- VernacularTitle:陷窝蛋白-1对永久性大脑中动脉闭塞小鼠缺血皮质白细胞介素-1β和白细胞介素-6表达的影响
- Author:
Sufen HUANG
;
Shengqiang ZHOU
;
Dong LUO
;
Jian YI
;
Baiyan LIU
- Keywords:
BrainIschemia;
Caveolin1;
Inflammation;
Interleukin-1β;
Interleukin-6
- From:
International Journal of Cerebrovascular Diseases
2016;24(11):1022-1027
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveToinvestigatetheeffectsofcaveolin1(Cav1)onexpressionsofproinflammatory cytokine interleukin (IL)-1βand IL-6 in the ischemic cortex after permanent middle cerebral artery occlusion in mice. Methods The Cav-1 knockout mice (n=40) and wild-type mice (n=40) were randomly divided into ischemia groups and sham operation groups (n=20 in each group). They w ere also redivided into ischemia or sham operation at 3, 7, 10 and 14 d time points ( n=5 in each time point). A permanent middle cerebral artery occlusion model w as induced by the suture method. Immunohistochemical method w as used to detect the expressions of IL-1βand IL-6 in the ischemic cortex. Results The expression levels of IL-1βand IL-6 in the ischemic cortex at each time point in the ischemia group in Cav1 knockout mice w ere significantly higher than those in the ischemia group in the w ild-type mice ( al P< 0.05 ). Conclusions The upregulations of proinflammatory cytokines IL-1βand IL-6 in the ischemic cortex in Cav1 knockout mice suggests that Cav1 plays an important role in aleviating inflammation after cerebral ischemia.
