Cartilage Engineering Using Fibrin Gel and Chondrocyte Cell Sheets
- VernacularTitle:应用纤维蛋白胶复合细胞膜片构建人工软骨
- Author:
Libin ZHOU
;
Bingxin XU
;
Ruiying DING
;
Haolun HAN
;
Gang WANG
;
Baowei LI
;
Hongnan WANG
;
Wei WU
- Keywords:
fibrin gel;
cell sheets;
cartilage engineering
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2017;38(1):122-127
- CountryChina
- Language:Chinese
-
Abstract:
Objective]To study the feasibility of Cartilage engineering using fibrin gel and chondrocyte cell sheets.[Methods]rabbit auricular chondrocytes were isolated and cultured to form cell sheets in flasks. The cell sheets were harvested using cell scrapers,and cut into fragments. The two precursor solutions of Fibrin gel were used to suspend the cell sheet fragments and isolated chondrocytes,and then added into the wells of a 48-well plate to form Gelatinous chondroid disc constructs. After in vitro culture, the constructs were implanted into nude mice. After 8 weeks,the constructs were harvested,and the specimens were evaluated using grossly observing, histological and immunohistochemical observation. [Results]Mature cartilage discs were obtained. The histomorphology of the explanted discs appeared non-uniform cartilaginous tissue comprise of regenerated cartilage islands with different size and irregular shape. Immunohistochemistry staining demonstrated that type II collagen highly expressed in the ECM of the cartilage islands. In 1 of the 8 discs,partial ossification was observed.[Conclusion]Fibrin gel is a favourable carrier. Artificial cartilage with stereochemical structure was constructed via combining the fibrin gel and chondrocyte cell sheets.
