Impact of High Thoracic Epidural Blockade on Autonomic Nerve Remodeling in Experimental Dogs With Atrial Fibrillation
10.3969/j.issn.1000-3614.2016.12.016
- VernacularTitle:高位胸段硬膜外阻滞对心房颤动自主神经重构的影响
- Author:
Yong CAO
;
Xiaoyan TIAN
;
Ran ZHANG
;
Yuchuan DAI
;
Jiaqi ZHAO
;
Yi AN
- Keywords:
Atrial ifbrillation;
Autonomic nerve remodeling;
Injection,epidural;
Lidocaine
- From:
Chinese Circulation Journal
2016;31(12):1219-1223
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To observe the impact of high thoracic epidural blockade (HTEB) on atrial autonomic nerve remodeling in dogs with atrial ifbrillation (AF) induced by long-term rapid right atrial appendage pacing and to explore the effect of nerve growth factor (NGF) on atrial autonomic nerve remodeling.
Methods: AF model was established by consistent rapid atrial pacing for 6 weeks. 18 experimental dogs were randomly divided into 3 groups: Sham group, the dogs had no pacing while received normal saline injection; Control group, the dogs had pacing and normal saline injection; HTEB group, the dogs had pacing and 0.5% lidocaine injection for HTEB.n=6 in each group. Atrial myocardium collagen volume fraction (CVF) was examined by Masson staining; sprouting of NGF related protein 43 (GAP43) and tyrosine hydroxylase (TH) were assessed by immunohistochemistry; protein expressions of NGF, GAP43 and TH were measured by Western blot analysis.
Results: Compared with Sham group, HTEB group showed decreased CVF and sprouting of GAP43, TH,P<0.05;reduced protein expressions of NGF, GAP43 and TH,P<0.05-0.01. Compared with Sham group, HTEB group presented increased CVF and sprouting of GAP43, TH,P<0.05-0.01; elevated protein expressions of NGF, GAP43 and TH,P<0.05.
Conclusion: Long-term rapid atrial pacing induced AF dog had inhomogeneous sprouting of atrial myocardial nerve which may cause autonomic nerve remodeling; NGF played the important role in such process. HTEB could effectively inhibit NGF up-regulation and suppress the autonomic nerve remodeling in experimental dogs.
