Effects of autophagy on the proliferation and migration of cervical cancer cells
10.11904/j.issn.1002-3070.2016.06.001
- VernacularTitle:自噬对宫颈癌细胞增殖和迁移能力的影响
- Author:
Yi YANG
;
Yan DING
;
Aiqing YU
;
Shirong YAN
- Keywords:
Autophagy;
Cervical cancer cells;
Rapamycin;
LC3B
- From:
Practical Oncology Journal
2016;30(6):481-486
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of autophagy on the proliferation and migration of cervi-cal cancer cells ,as well as the underlining mechanisms .Methods Rapamycin was used to induce the autophagy in HeLa cells,formation of autophagosomes was observed by staining with acridine orange under fluorescence mi -croscope.Western blot was used to detect the expression of LC 3 and PI3K/Akt/mTOR in HeLa cells.The LC3 plasmid was transfected into HeLa cells .The distribution of LC3 in cells and the expression of LC3 was identified by fluorescence microscope and Western blot ,respectively.The autophagy was inhibited with 3-methyladenine(3-MA )in HeLa cells.The cell proliferation was monitored by RTCA real -time instrument.Transwell chamber was carried out to assess cell migration .Results After 6h of rapamycin treatment ,the expression of LC3B was in-creased in HeLa cells ( P<0 .05 ) .The proliferative and migration ability were weakened compared to wild type HeLa cells(P<0.05).The same results in the presence of 3-MA.The expression of PI3K/AKT/mTOR path-way proteins were activated by rapamycin treatment and LC 3 overexpression(P<0.05).Conclusion Autophagy can suppress the proliferation and migration in cervical cancer cells ,which may relate to PI3K/Akt/mTOR path-way.
