Experimental research of inhibitory effects of garlic active ingredients DATS on PAF-mediated melanoma metastasis
10.3969/j.issn.1001-1978.2016.12.008
- VernacularTitle:大蒜活性成分DATS抑制血小板激活因子介导的黑色素瘤转移的实验研究
- Author:
Ying SHEN
;
Yuping LIU
;
Xu WANG
;
Peiliang SHEN
;
Pingting ZHU
;
Wenxing CHEN
;
Aiyun WANG
;
Yin LU
- Keywords:
allicin;
melanoma;
tumor metastasis;
DATS;
PAF;
MAPK
- From:
Chinese Pharmacological Bulletin
2016;32(12):1670-1676
- CountryChina
- Language:Chinese
-
Abstract:
Aim To discuss the impact of important ac-tive ingredient of garlic diallyl sulfide———DATS on platelet activating factor (PAF ) mediated melanoma metastasis and its mechanisms.Methods ①MTT was used to test the effect of different concentrations of DATS on B16F10 and A375 melanoma cell growth number;②Scratch test and transwell were employed to test the effect of different concentrations of DATS on B16F10 and A375 melanoma cell migration;③ West-ern blot was used to test the effect of DATS on expres-sion of MMP-2,ERK,p38 induced by PFA;④Intrave-nous injection of tumor metastasis model was used to check the inhibition of DATS in PAF-mediated melano-ma metastasis.Results B16F10 cells relative growth rate fell to 73.21% and 48.78%,respectively,when DATS concentration reached 50 and 100 μmol·L-1 . DATS inhibited the levels of PAF-induced migration of melanoma cells B16F10 and vertical migration signifi-cantly,and inhibited B16F10 cells migration induced by PAF through inhibiting the expression of MMP-2, paxillin protein,FAK and other proteins.Conclusion DATS can significantly inhibit PAF-induced tumor metastasis, which is related to the inactivation of MAPKs.
