Ribonucleic acid Ⅱ induces apoptosis in human leukemia cells by up-regulating p53
10.3969/j.issn.1001-1978.2016.12.018
- VernacularTitle:注射用核糖核酸Ⅱ上调p53诱导人白血病细胞凋亡
- Author:
Pei GUO
;
Jianhua RAN
;
Jing LI
;
Dilong CHEN
;
Baoxue YANG
;
Fei HE
;
Wei XIONG
;
Xueping SHI
;
Haixing LI
- Keywords:
ribonucleic acid Ⅱ;
leukemia;
p53;
K562 cells;
KG1 a cells;
apoptosis
- From:
Chinese Pharmacological Bulletin
2016;32(12):1729-1734
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effect of ribonucleic acidⅡon apoptosis in human leukemia cell lines K562 and KG1 a.Methods Cell counting kit-8(CCK-8)as-say was performed to detect proliferation activity of K562 and KG1 a cells treated with ribonucleic acidⅡ. Apoptosis index was assessed by flow cytometry(FCM) and fluorescent Hoechst 33258 staining was used for observing morphologic changes of apoptosis.Expres-sion levels of p53,Bax,Bcl-2 and cleaved caspase-3 were analyzed by Western blot.Results The prolifera-tion of K562 and KG1 a cells was significantly inhibited by ribonucleic acid Ⅱ treatment for 12 h,24 h,48 h at concentrations of 100~300 mg·L-1 ,which indica-ted the inhibitory effect of ribonucleic acid Ⅱ was in dose-dependent and time-dependent manners.FCM re-sults displayed a dose-dependent increase in cell apop-totic rate.Hoechst 33258 staining showed the typical apoptotic morphology in some leukemic cells treated with ribonucleic acid Ⅱ,including increased nuclear chromatin concentration and edge accumulation.West-ern blot analysis showed the increased expression of p53,Bax,cleaved caspase-3 and decreased expression of Bcl-2 in K562 and KG1 a cells treated with ribonu-cleic acid Ⅱ.Conclusions Ribonucleic acid Ⅱ can induce apoptosis of leukemia K562 and KG1 a cells by up-regulating p53,which mediates Bcl-2/Bax balance and activates caspase-3 .
