Characteristics of stem cell spheres of the renal carcinoma cell lines SN12C and 786-O grown in serum-free culture medium
10.3969/j.issn.1000-8179.2016.23.071
- VernacularTitle:肾癌细胞系SN12C和786-O无血清培养的干细胞球特性的比较
- Author:
Yanhui ZHANG
;
Baocun SUN
;
Xiulan ZHAO
;
Zhiyong LIU
;
Xin YAO
;
Xueyi DONG
- Keywords:
renal cell carcinoma;
cancer stem cell;
SN12C;
786-O
- From:
Chinese Journal of Clinical Oncology
2016;43(23):1021-1025
- CountryChina
- Language:Chinese
-
Abstract:
Objective:This study aims to determine the suitable cell line to be used in isolating cancer stem cells by comparing the characteristics of tumor stem cells in renal cell carcinoma cell lines SN12C and 786-O. Methods:The rate of sphere formation in SN12C and 786-O cells was determined in serum-free medium (SFM). The expression levels of CD133, CD44, Nanog, and Oct3/4 were investi-gated through flow cytometry. Moreover, the tumorigenicity of spheroid cell that originated from SN12C and 786-O cells was investi-gated in vivo by using a tumor model. Results:The average time of sphere formation in SFM was shorter in SN12C than in 786-O (5 days vs. 7 days). Moreover, the expression levels of CD133, CD44, Nanog, and Oct3/4 in SN12C and 786-O significantly differed (P<0.05). When transplanted in nude mice, 786-O spheres were less tumorigenic than SN12C spheres. Conclusion:SN12C spheres possess the main defining characteristics of renal cancer stem cell;thus, SN12C is the more suitable cell line to be used to isolate cancer stem cells compared with 786-O.
