Effect of Naoxintong on nuclear transcription factor -κB, matrix metalloproteinase-9 and TNF-αin the brain of ischemic rat models
- VernacularTitle:脑心通对缺血性大鼠脑组织核转录因子-κB、基质金属蛋白酶-9和肿瘤坏死因子-α的影响
- Author:
Ming YU
;
Yanling LUO
;
Haitao ZHU
- Keywords:
nuclear transcription factor-κB;
matrix metalloproteinase-9;
TNF-α;
inflammation;
Naoxintong
- From:
Journal of Clinical Neurology
2016;29(6):447-450
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of Naoxintong on nuclear transcription factor-κB ( NF-κB ) , matrix metalloproteinase-9(MMP-9)and TNF-αin the brain of ischemic rat models.Methods One hundred and ten healthy male SD rats were randomly divided into sham operated group ( n=30 ) , model control group ( n=40 ) and Naoxintong treatment group(n=40).Each group was further divided into five sub group by 0.5d, 1 d, 3 d, 5 d and 7 d after ischemia-reperfusion.The middle cerebral artery occlusion ischemia reperfusion rat models were prepared by Zea Longa method;meanwhile the MRI and the Bederson score were used to select the successful models .In order to observe the changing process of the ischemic brain tissue after given Naoxintong capsule , six rats in each group each timepoint were examined by MRI .The expression levels of protein and mRNA of NF-κB, MMP-9 and TNF-αwere detected respectively by western blot and real time PCR .Results Cerebral infarction volume of Naoxintong treatment group was significantly reduced compared with the model control group .The mRNA and protein levels of NF-κB, MMP-9 and TNF-αof the Naoxintong treatment group decreased significantly compared with the model control group (P <0.05).The mRNA and protein levels of NF-κB, MMP-9 and TNF-αhad no significant difference between the sham group and Naoxintong treatment group .Conclusion Naoxintong has a neuroprotection effect on cerebral ischemia via alleviating the inflammatory factors in the ischemic area .
