Effects of Ursolic Acid on Cholesterol Metabolism in Hepatic Cells
10.3870/j.issn.1004-0781.2017.01.002
- VernacularTitle:熊果酸对肝细胞胆固醇代谢的影响
- Author:
Kuiying LIANG
;
Xia CHU
- Keywords:
Ursolic acid;
Cholesterol metabolism;
Cholesterol 7alpha-hydroxylase
- From:
Herald of Medicine
2017;36(1):9-12
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects and mechanism of ursolic acid ( UA) on cholesterol metabolism in human hepatocellular carcinoma HepG2 and mouse hepatocyte AML-12. Methods HepG2 and AML-12 cells were treated with different concentrations of UA (0,10,20,40 μmol·L-1)for 24 h, then the mRNA and protein expression of cholesterol 7alpha-hydroxylase (CYP7A1) and intracellular cholesterol level was detected by RT-PCR、Western blotting and enzymatic method, respectively. Results Compared with 0 μmol·L-1 UA, 20 μmol·L-1 and 40 μmol·L-1 UA significantly increased the expressions of CYP7A1 mRNA and protein(P<0. 05), and decreased intracellular cholesterol level in HepG2 and AML-12 cells (P<0. 05). Conclusion A certain concentration of UA can reduce the level of cholesterol in HepG2 and AML-12 cells. CYP7A1 may be involved in the regulation process.
