Prevention of 2 ,3 ,5 ,4 '-Tetrahydroxystilbene-2-O-beta-D-Glucoside on Atherosclerotic Plaque Formation in ApoE Knock-out Mice
10.3870/j.issn.1004-0781.2017.01.003
- VernacularTitle:二苯乙烯苷对载脂蛋白E基因敲除小鼠动脉粥样硬化的防治作用
- Author:
Youzhi ZHANG
;
Qi HUANG
;
Qing MIN
;
Qingchuan LIAO
;
Fei CAI
- Keywords:
2;
3;
5;
4'-tetrahydroxystilbene-2-O-beta-D-glucoside;
Lectin-like oxidized low-density lipoprotein receptor-1;
ApoE knock-out;
Atherosclerosis
- From:
Herald of Medicine
2017;36(1):13-16
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the protective effect and mechanism of 2,3,5,4 '-tetrahydroxystilbene-2-O-beta-D-glucoside ( THSG) on atherosclerosis in ApoE konck-out mice. Methods A total of 24 ApoE knock-out mice were randomly divided into normal control group (n=8), model control group (HFD, high-fat diet, n=8) and treated group (THSG, 20 mg· kg-1, i. g. , n=8). The atherosclerosic plaque of aorta wall and aorta root were measured by oil red O staining;The expression of lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) in human umbilical vein endothelial cells (HUVEC) through C-reaction protein ( CRP) was studied by Western blotting. Results The atherosclerosis plaque in normal control group was not observed. The lipid accumulation decreased in the aorta and the plaque areas in the aortic sinus in THSG treated-group compared with model control group. Moreover, THSG down-regulated CRP-induced LOX-1 expression in HUVEC. Conclusion The atheroscletosis plaque in ApoE knock-out mice was decreased by THSG. The mechanism might be related to the inhibition of the expression of LOX-1 protein.
