Effect of Zingiber corallinum Oil on Proliferation and Apoptosis of Cervical Carcinoma Cell Line HeLa

VernacularTitle:珊瑚姜油对宫颈癌HeLa细胞增殖及凋亡的影响
Author: Chunwei WU ; Yunhua TU ; Minge LI ; Zhenyuan YE ; Yuecui XUE ; Yu CAO
Keywords: Zingiber corallinum oil; Carcinoma; cervical; HeLa cell; Cell cycle
From: Herald of Medicine 2017;36(1):32-36
CountryChina
Language:Chinese
Abstract: Objective To investigate the effect of Zingiber corallinum oil ( ZCO ) on apoptosis and proliferation of cervical carcinoma cell line HeLa. Methods HeLa cells were treated with different concentrations of ZCO(5-80 mg·L-1)in vitro. Cytotoxicity rate was determined by CCK-8 assay. The morphological changes was observed using inverted microscope after AO/EB staining. Caspase-3 activities were measured with a colorimetric method. Protein level of hsp-70 were detected by Western blotting. Cell cycle and apoptosis were analyzed by flow cytometer ( FCM ) . Results ZCO exhibited effect of proliferation inhibition and apoptosis-inducing on the growth of HeLa cells in a dose-dependent manner. Caspase-3 activities increased in a dose-dependent manner while the expression of hsp-70 decreased. Cell cycle was arrested in G2/M phase. Conclusion ZCO exhibites a marked effect of proliferation inhibition and apoptosis-inducing on HeLa cells. The mechanism of ZCO might be activating the key enzyme in apoptotic pathway, so that the expression of hsp-70 is down-regulated, and cell cycle is arrested in G2/M phase.