Determination of Four Flavonoids in Shenyan Siwei Granules by Quantitative Analysis of Multi-components with Single Marker
10.3870/j.issn.1004-0781.2017.01.016
- VernacularTitle:一测多评法测定肾炎四味颗粒中4种黄酮类成分的含量
- Author:
Wei ZHOU
;
Quansheng CAO
- Keywords:
Shenyan siwei granules;
Baicalin;
Wogonoside;
Baicalein;
Wogonin;
Quantitative analysis of multi-components with single marker;
Relative correction factor;
Chromatography;
high performance liquid
- From:
Herald of Medicine
2017;36(1):65-69
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish an HPLC method to measure four flavonoids ( baicalin, wogonoside,baicalein and wogonin) in shenyan siwei granules by quantitative analysis of multi-components with single marker ( QAMS ) . Methods Agilent ZORBAX SB-C18 column(4. 6 mm × 250 mm,5 μm) was used. The mobile phase was composed with methanol and 0. 4% phosphoric acid solutionat at 1. 0 mL·min-1 flow rate with gradient elution. The detection wavelength was 278 nm. Baicalin was used as the internal reference substance. The relative correction factors ( RCF) between the baicalin and the other three flavonoids were established to detect the quantitation of baicalin and calculate the quantitation of the other three constituents. The external standard method was used for quantitating the four constituents, and the method was evaluated by comparing to the quantitative results between external standard method and QAMS method. Results The results of QAMS method had no significant difference with those of external standard method. Conclusion It is feasible and accurate to control the quality of shenyan siwei granules with QAMS.
