Simultaneous Determinations of Four Ingredients in Yiqi Hewei Capsules by HPLC
10.3870/j.issn.1004-0781.2017.01.017
- VernacularTitle:高效液相色谱法同时测定益气和胃胶囊中4种成分的含量
- Author:
Qingbing CHENG
;
Xiaomei XU
- Keywords:
Yiqi hewei capsules;
Baicalin;
Hesperidin;
Naringin;
Neohesperidin;
Chromatography;
high performance liquid
- From:
Herald of Medicine
2017;36(1):70-72
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for the quantitative determinations of the ingredients in yiqi hewei capsules. Methods RP-HPLC method was used. The separation was performed on a Thermo C18 column with mobile phase consisting of acetonitrile-water (20: 80) (pH was adjusted to 3 with glacial acetic acid) at the flow rate of 1. 0 mL·min-1. The detection wavelength was 283 nm. Results The linear ranges were ( 0. 5735 -5. 7360) × 10 -2 mg·mL-1 for baicalin, (0. 1940-1. 9395) × 10 -2 mg·mL-1 for hesperidin, (0. 2051 -2. 0510) × 10 -3 mg·mL-1 for naringin and (0. 2020 -2.0200) ×10 -2 mg·mL-1 for neohesperidin;RSD =0.28%,0.54%,0.54%,0.31%(n =5),respectively. The average recoveries were 103. 94%, 98. 06%, 103. 09%, 95. 67%, respectively. Conclusion The established method is simple, sensitive and accurate,which can be used for the quality control of yiqi hewei capsules.
