Construction and screening of RPL23-siRNA interference fragments
10.11904/j.issn.1002-3070.2016.06.002
- VernacularTitle:RPL23－siRNA干扰片段的构建及筛选
- Author:
Wenmiao PENG
;
Zhimin ZHANG
;
Meng HU
;
Lifang YU
;
Bicheng ZHANG
;
Zhiguo RAO
;
Chuanrong QIN
- Keywords:
RPL23;
siRNA;
RNAi
- From:
Practical Oncology Journal
2016;30(6):487-491
- CountryChina
- Language:Chinese
-
Abstract:
Ob jective To construct and screen out the RPL 23-siRNA interference fragments ,providing the basis for the following experiments about the correlation with RPL 23 and gastric cancer .Methods The RPL23-siRNA,synthesized chemically through lipofection ,were selected from three target sequences by RNA in-terference and detected by real -time PCR and Western blot .Results Compared with normal cell group and RPL23 control group ,the mRNA and protein expression of RPL 23 in the other 3 interference groups were signifi-cantly decreased(P<0.01).Multiple comparisons showed that the interference efficiency of RPL 23 -siRNA1 group was significantly higher than that of RPL 23-siRNA2 group and RPL23-siRNA3 group(P<0.01).Con-clusion The RPL23-siRNA interference fragment can be successfully constructed and screened out ,which pro-vides the basis for the following experiments .
