Investigation on reference interval of serum non-esterified fatty acid among population in Wuhan area
10.3969/j.issn.1673-4130.2016.23.004
- VernacularTitle:武汉地区人群血清游离脂肪酸参考区间的探索
- Author:
Yan ZHOU
;
Pei MA
;
Lu HAN
- Keywords:
enzymatic assay;
serum non-esterified fatty acids;
reference interval
- From:
International Journal of Laboratory Medicine
2016;37(23):3249-3251
- CountryChina
- Language:Chinese
-
Abstract:
Objective To preliminarily establish the reference interval of serum non‐esterified fatty acids(NEFA)among popula‐tion in Wuhan area by enzymatic assay .Methods NEFA level of serum samples from A total of 1 250 individuals undergoing healthy physical examination in our hospital were selected as the research subjects .The Olympus AU5400 biochemistry analyzer and SEKISUI reagents were adopted to detect serum NEFA level .The regression analysis was adopted to analyze the NEFA influencing factors .The NEFA 95% reference interval was estimated by using the normal distribution method and the reference interval was verified .Results The regression analysis showed that the body mass index (BMI)and age had significant effect on NEFA (P<0 .01) .NEFA was highest in the group aged ≥60 years old ,followed by the group of 18- <45 years old ,and lowest in the group of 45-60 years old ;NEFA was highest in the group of BMI<18 .5 kg/m2 .The 95% reference interval of NEFA for healthy popula‐tion in Wuhan area was estimated as 204 .6 -975 .2 μmol/L .The detected NEFA level for clinical verifiers was 242 .0 -831 .2μmol/L ,which conformed to the above reference interval .Conclusion The serum NEFA reference interval among healthy popula‐tion in Wuhan area is primarily established .
