Prostaglandin E2 induced myofibrolast phenotype inversion and interleukin 8 expression in prostate stromal cells
10.3969/j.issn.1673-4130.2016.23.023
- VernacularTitle:前列腺素 E2诱导前列腺间质细胞肌成纤维细胞表型转化和白细胞介素-8的表达
- Author:
Ju ZHANG
- Keywords:
mesenchymal cell activation;
immunofluorescence staining;
myofibroblast phenotype
- From:
International Journal of Laboratory Medicine
2016;37(23):3300-3302
- CountryChina
- Language:Chinese
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Abstract:
Objective To investigate the effects of prostaglandin E2(PGE2) in prostate mesenchymal cell activation .Methods To culture human prostate stromal cells WPMY‐1 in vitro ,implement immunofluorescence staining after treating with DMSO and 10-9 mol/L PGE2 and detect the change of the myofibroblast phenotype .The expression of cellular inflammatory factor interleukin 8(IL‐8) was detected by real‐time quantitative PCR and the concentration of IL‐8 was detected .Results PGE2 significantly in‐creased the cellular proportion of colocalization with alpha SMA and Vimentin in WPMY‐1 cells .PGE2 promoted the expression of IL‐8 in WPMY‐1 cells .Conclusion PGE2 can increase myofibroblast phenotype in human prostate mesenchymal cells WPMY‐1 in vitro culture ,promotes the IL‐8 expression ,which has an important role in the occurrence and development of prostate cancer .
