Effects of diterpene ginkgolides meglumine injection on neurotransmitters in cerebral ischemia-reperfusion injury rats

VernacularTitle:银杏二萜内酯对缺血／再灌注大鼠脑组织中神经递质的影响
Author: Wen ZHANG ; Junke SONG ; Guorong HE ; Xue ZHANG ; Qimeng ZHOU ; Zhenzhong WANG ; Wei XIAO ; Zhiyong XIAO ; Wenxia ZHOU ; Guanhua DU
Keywords: DGMI; cerebral ischemia-reperfusion; a-mino acid neurotransmitters; monoamine neurotrans-mitters; HPLC-ECD; metabonomics
From: Chinese Pharmacological Bulletin 2016;32(12):1648-1656
CountryChina
Language:Chinese
Abstract: Aim To investigate the effects of diterpene ginkgolides meglumine injection (DGMI ) on amino acids and monoamine neurotransmitters in rats with cerebral ischemia/reperfusion injury.Methods In-traluminal suture was applied to establish middle cere-bral artery occlusion (MCAO/R)model with ischemia for 1.5 h and reperfusion for 24 h.After the adminis-tration of DGMI (i.v.),the levels of amino acid and monoamine neurotransmitters in brain tissue were de-tected through HPLC-ECD.Results DGMI down-reg-ulated the concentrations of aspartic acid, glutamic acid,glycine and γ-aminobutyric acid which were in-creased in MCAO/R group.DGMI also reduced the levels of norepinephrine epinephrine,glyoxylic acid, serotonin and 5-HIAA in cortex and hippocampus,and increased adrenaline content compared to the model group.Conclusion DGMI exhibits a protective role in rats with cerebral ischemia /reperfusion injury through regulating amino acids and monoamine neuro-transmitters.